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It is tempting to suggest, based on our results, that the species populated at high pH is also a molten-globule-like species, although it did not bind ANS (Fig
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. Structure and confomiational stability of the enzyme I of Slreplomyces coelicolor explored by KMR and circular dichroism
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. On the contrary, an important consequence of creating an internal cavity of 40 in I7S and 100 [Angstrom]^sup 3^ in F110S, presumably filled with water (18,19), is the onset of large amplitude structural fluctuations that increase remarkably the flexibility of the globular fold, as evidenced by the three to four orders of magnitude increase in permeability to acrylamide (18)
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