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Denaturing behavior of glutathione reductase from cyanobacterium Spirulina maxima in guanidine hydrochloride
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. Calorimetric denaturations were reversible in all cases
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. 2% collagenase (Sigma, type I, St
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. Although glycosylation does not affect the structure, protein dynamic fluctuations decrease globally, decreasing amide hydrogen exchange rates by up to sixfold throughout the entire protein
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. Another report has demonstrated that Na+ ion nearly doubled the rate of mitosis in cells (Atkinson et al
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. Lipids were mixed in chloroform and dried to a thin film in a small pear shaped flask using a rotary evaporator
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. This probe becomes fluorescent when it reacts with cysteine side chains (see Molecular Probes Handbook, Eugene, OR)
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. , 2003a), show that the heat- and denaturant-induced transitions in discoidal lipoproteins are kinetically controlled reactions that involve protein unfolding, dissociation, and lipoprotein fusion
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. 6 and 7 B) differs from the stability ranking apoA-2 > apoA-1 that is inferred for plasma spherical HDL and is attributed to deeper penetration of the more hydrophobic apoA-2 ?-helices in the lipid milieu (Brasseur et al
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. These naturally belong to the levels of coarse-graining at which we treated the helix bundles above
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. For the WT ribozymes, docked fractions (F) were calculated as a ratio of docked/undocked areas (A) as, F = A^sup docked^/(A^sup docked^ + A^sup undocked^)(Deniz et al
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- For example, concanavalin A (Con A) shows a stability of 30-32 kcal/mol, and under identical conditions SBA shows a stability of 58 kcal/mol
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.) the ensemble of unfolded states is characterized by diverse structural and conformational properties
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. This collective process could explain the fact that essentially all resonances affected by conformational exchange have approximately the same time constant and are clustered in regions important for either CaM:CaM or CaM:SEF2-1mp interactions
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