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In line with these results, the presence of MG intermediate states in the unfolding of S
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. As a consequence the obtained transition temperatures are strongly depending on the heating rates (for a more extended discussion on this subject see (17))
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. However, our data indicate that aggregation and in addition at least one of these other mechanisms seems to play a role for the ?-amylases with red shifts upon thermal unfolding (i
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. © 2006 by the Biophysical Society 0006-3495/06/03/1797/07 $2
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. (15) and references therein), MD simulations (16), and incoherent neutron scattering (17)
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. Such work is ongoing in our laboratories, and data from misfolded TTR oligomers recorded from site-specific fluorescence labeling of cysteine mutants have provided size distributions with average rotation correlation times of ~ 150 ns (K
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. Taken together, the smooth E shifts with changing Mg^sup 2+^ concentration combined with the observed broadening/ tailing at high Mg^sup 2+^ concentration are consistent with the presence of a dynamic equilibrium between the quasi-docked state and an extended undocked state, with a relatively small energy barrier for interconversion (Fig
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- Steady-state and quenching experiments were acquired at 298 K
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. Stabilization of recombinant Drosophila acetylcholinesterase
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