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Using these procedures, the energetics and pathways of folding, as well as the existence of transient intermediate states between unfolded and fully folded polypeptides, have been characterized for a number of proteins (for a collection of reviews, see Creighton, 1992; Pain, 1994)
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. Iodide is a negatively charged quencher expected to quench the fluorescence emission of solvent-exposed tryptophan residues (Lakowicz, 1983)
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. It can retain its activity when subjected to heat treatment at 60°C for 60 min (25), whereas it is irreversibly unfolded when heated at temperatures above 65°C
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. Perturbation of protein tertiary structure in frozen solutions revealed by 1-anilino-8-naphthalene sulfonate fluorescence
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. Although the wraparound mode of target binding is the most studied, alternative binding modes have become evident
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. Although the misfolding and aggregation reaction is very complex and of multiple order, all kinetic traces could be fitted to a double exponential function which enabled comparison of kinetic parameters
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. Mature human plasma HDL and LDL are also stabilized by kinetic barriers, suggesting that such barriers provide a universal natural strategy for lipoprotein stabilization (Mehta et al
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. Scavenger receptor class B, type I-mediated uptake of various lipids into cells
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. Therefore, throughout the following overnight dry incubation period no further structural transitions are likely to occur in the amyloid samples
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. ) Idler EL, Russell LB, Davis D
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. Treatment did increase the belief that others can help with the drug problem, but it did not improve drug-resistance self-efficacy
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. "Ask yourself, quite simply, whether you've received the service you expected," says Sebastian Moser, U
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. However, the technology on which those drugs are based came from outside the company
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. 4 billion in 2002, they fell to only $1
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. Principles of forensic toxicology
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. the true acquisition cost to the community pharmacist
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. Diet and trophic characteristics of Great Horned Owls in southwestern Idaho
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. Conflicts of interest emerge from many directions
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. In a 2003 report in Pharmacology, Biochemistry and Behavior, daily electrical shocks left rats with cognitive deficits, suppressed immune systems, and high blood levels of the stress hormone corticosterone
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. Social support has been associated with increased health, mental well-being, and longevity (9, 10)
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. Studies have found that associations with drug users are related to a higher risk of getting involved with drugs (31)
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. Each valve had "stuck-on" plaques comprised of myofibroblasts in a myxoid matrix
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. According to adverse event reports (AERs) submitted to the FDA,6 59% used ephedrine-type alkaloid products for weight loss, 16% to improve athletic performance, and 6% to increase energy
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- Doubly distilled milliQ water was used throughout
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.22 µm Millipore (Billerica, MA) filter into a quartz cuvette and maintained at 25°C
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.EFFECT OF MELT FLOW RATE ON TONER PENETRATIONThe behavior of the toner on the substrate depends on the curing temperature
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.The reactivity of the antibodies produced by the hybridomas was then determined
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. The cleavage reaction was initiated by addition of substrate strands (500 pM) to the respective preformed ribozyme complex (100 nM) at room temperature and reaction progress was monitored by gel analysis of aliquots quenched with formamide at different time points
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. In addition to predicting adult drug problems, adolescent substance use in females has been found to predict delinquent behavior and theft in adulthood (Kandel et al
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."Perhaps the biggest process-change issue that clients face is that the data must be shared not just within the company in ways that it wasn't before, but more importantly across the supply chain
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. Note: RFC stands for Request for Comment
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. Great Horned Owls, in particular, show a propensity to crush the bones of their prey prior to ingestion, making them more susceptible to chemical dissolution (Dodson and Wexlar 1979, Duke et al
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.Samples for transmission electron microscopy were fixed in 0
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