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MATERIALS AND METHODS Materials Horse apomyoglobin was prepared by using Hapner's method (Hapner et al
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. The +4°C shift, the reduced height, and the increased width (decreased cooperativity) of this peak as compared to the sharp transition in DMPC vesicles at 24°C (Chapman, 1975) are characteristic of lipoprotein disks that have smaller co-operative lipid domains than vesicles
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