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The thermal dependence of ACYI activity was measured by incubating the enzyme solutions at given temperatures for 30 min, and then the activity assay was performed by mixing the enzyme solutions and reaction buffers preheated at the same temperatures
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. This is called "wraparound" binding because the two domains in CaM wrap around the ?-helical target (8,9)
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. Only residues 36, 42, 57, and 115 are exceptions with k^sup -1^^sub ex^ values above ~200 µs
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. The Part B model coefficient changed from -2 to +2, p<
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- For indole in aqueous solution, k^sub q^ for iodide quenching is 6
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.0) and in all cases, the intensity of the fluorescence at 480 nm was very high at low temperatures (between 288 and 293 K)
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. On the red edge of the emission spectra a shoulder is visible for thermal unfolded states but not for the native and the GndHCl-induced unfolded states
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.The variation in (?^sup o^^sub m^)^sub app^ in Fig
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. Osmotic equilibria in solutions of serum albumin and sodium chloride
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.XPCOM is a feature of the Mozilla environment that allows developers to perform lower-level tasks that cannot be done in XUL or JavaScript
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. N Engl J Med 1994:331:889-95
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.Conflicts of interest emerge from many directions
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. If significant differences are present in the pregain coefficients, then an additional step is needed to control for them
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