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34^ for folded proteins (66), the scaling law itself does not restrict folded proteins to be spherical globules
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- Similar results have been observed in 1), the enzyme malate synthase G (54), a close structural analog of pyruvate dikinase and EIC (11), where binding to pyruvate does not cause large structural changes (55); and 2), the pyruvate dikinase from maize, where only small changes are observed in selected regions upon binding of PEP (56)
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. From the often drastic shortening of the phosphorescence lifetime of Tip residues buried within the rigid core of these macromolecules, it was deduced that their internal flexibility is greatly enhanced in ice, a response to freezing similar to that observed when the polypeptide loses much of its tertiary structure as in the formation of molten globule states
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. Using ^sup 31^P chemical shift differences or lineshape analysis of ^sup 14^N ammonium, cytoplasmic and vacuolar regions in maize root tissue could be distinguished (21,22)
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