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To characterize in more detail the structural changes induced by GdnHCl, we have investigated the accessibility of thiol groups of GR (which contains six Cys residues per subunit) to modification with DTNB (Fig
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Aminoacylase activity assay The enzyme was dissolved in 30 mM Tris-HCl, pH 7
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The R variable is a group indicator = 1 for Gainers and 0 for Nevers
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