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Principles of Polymer Chemistry
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- Titration of reactive thiol groupsThis was performed by following the increase in 2-nitro-5-thiobenzoic acid (TNB) absorbance at 412 nm, according to Collier (1973)
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. In addition, using the simple general equation, which covers for both fast and slow exchange, derived by Ishima and Torchia (34), we also show that the exchange rate can be determined from a combination of CPMG measured R^sub ex^ and change in R^sub ex^, (?R^sub ex^, vide infra) without prior assumption on the exchange timescale
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. The data presented in Fig
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