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Interestingly, no significant spectral changes were detected up to 1 M GdnHCl, whereas an increase in reactivity of one thiol group of GR to DTNB was observed at this concentration of GdnHCl (Fig
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. This work was supported by the Research-Infra Construction Program and the National Research Laboratory Program (grants to C
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. Polyethylene glycols with molecular weights of 3000 and 10000 were purchased from Merck (Hohenbrunn, Germany)
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. Using the hairpin ribozyme as a model system, we specifically address a key question in RNA folding-how do preformed secondary structure elements dictate the course of subsequent tertiary folding events? Currently, little is known about how secondary structural elements affect the ribozyme conformational distributions important in the course of folding
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- Variability in quaternary association of proteins with the same tertiary fold: a case study and rationalization involving legume lectins
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. The intrinsic quenching of molecular rotors makes them sensitive to intrinsic rotational relaxation, which is slowed down during binding to aggregated protein
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. In addition, slower multiphase unfolding is detected at higher denaturant concentrations
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