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The IR and fluorescence results strongly suggested that the thermal unfolding of ACYI could be illustrated as a two-stage process involving an intermediate: native state (N) [arrow right] intermediate stale (I) [arrow right] aggregates (A)
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. Kinetics of aggregation and gelation of globular proteins after heat-induced denaturation
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. Several general predictions result: 1), intermediate subspaces of configurations will always be visited; 2), specific but nonnative interactions may be important in stabilizing these low-dimensional diffusive searches on the folding pathway, as well as native interactions; 3), sequential barriers will commonly be found, even in "two-state" proteins; 4), very early times will show characteristic departures from single-exponential kinetics; and 5), contributions of nonnative interactions to ?-values and "Chevron plots" are calculable, and may be significant
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. The Task Force report also underlines the agency's belief that widespread use of electronic pedigrees (e-pedigrees) using electronic track and trace technology, including radio frequency identification (RFID), would provide an electronic safety net for our nation's drug supply
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- Then, one is tempted to ask, is there any relationship among the different functions and structures of the PTS proteins on one side, and the thennodynamic parameters and folding properties on the other? To answer this question, it is necessary to describe the conformational preferences of proteins, where studies of different members of the family have been described
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. The probe DCVJ is a molecular rotor containing a fluorescent julolidine moiety and an intrinsic dicyano quencher
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