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Measuring unfolding of proteins in the presence of denaturant using fluorescence correlation spectroscopy
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Here, we have used high hydrostatic pressure to determine the volume change association with these interactions
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We find that membrane binding of PLCß, which is a chimera of PLCß^sub 2^ and PLC?^sub 1^, is pressure independent as expected from the pressure behavior of wild-type PLC?^sub 1^ (Rebecchi et al
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2, curves 1-4) indicates that this transition is invariant regardless of the nature of the complex (intact disks in the first scan or a mixture of vesicle-bound and monomolecular lipid-poor apoA-2 in the second and consecutive scans)
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For a molecule existing in two different states but with similar atom-positional fluctuations, similar configurational entropies are expected for the two states
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If successful, what strategies did the patient use to achieve the goal? If unsuccessful, what things got in the way, and how can they be removed in the future? Patients benefit more from examining how behavior changed or did not change rather than focusing on why things did not go as planned
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Parental and peer influences on the onset of heavier drinking among adolescents
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8 months, range 2 weeks to 36 months)
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- In addition, the existence of a partially denatured predissociated state has been proposed in the unfolding of the dimeric DNA-binding protein Arc represser (Peng et al
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. GndHCl-induced unfolded states show for all ?-amylases emission spectra with ?^sub max^ of ~348-349 nm which is nearly the value one would expect for fully accessible tryptophan, namely 350 nm
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.Timing of parturition was estimated by microscopically determining embryo development stages for all females with fertilized eggs following Yamada and Kusakari's (1991) stages of embryonic development for kurosoi (Sebastes schlegeli) modified to reflect the gestation period of 37 days for black rockfish (Boehlert and Yoklavich, 1984)
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.0001), and were more likely to be of Hispanic or mixed/other ethnicity, while the outpatient participants were more likely to be caucasian or African American ([[chi square]
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.Received January 13, 2004; revision received July 15, 2004; accepted August 20, 2004REFERENCESBachman, J
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. Note: RFC stands for Request for Comment
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. Gainetdinov RR, Wetsel WC, Jones SR, and others
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