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To better understand the step-wise changes of the different Trp fluorophores, the spectrum recorded at each temperature was fitted using the discrete states model of Trp residues in proteins (30,32,34)
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- The slit widths were 3 and 5 nm for emission and excitation, respectively
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. The distributions of distances are sharply peaked for near-neighbor residues and they become increasingly broad as sequence separation increases
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. We examined two single point cavity-forming mutants, I7S and F110S, whose internal cavities of 40 and 100 [Angstrom], respectively, appear to be fully hydrated (18), and the double mutant C3A/C26A in which the disulfide bridge linking strands ß1 and ß2 has been severed
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. Seung-Cheol Lee,* Jee-Hyun Cho,* Daniel Mietchen,*[dagger][double dagger] Young-Sook Kim,* Kwan Soo Hong,* Chulhyun Lee,* Dongmin Kang,§ Ki Deok Park,¶ Byong-Seok Choi,|| and Chaejoon Cheong* * Division of Proteome Research, Korea Basic Science Institute, Yusung-gu, Daejeon 305-333, Korea; [dagger] Fraunhofer Institute for Biomedical Engineering, Department of Magnetic Resonance, Sankt Ingbert, Germany; [double dagger] University of the Saarland, Faculty of Physics and Mechatronics, Saarbrücken, Germany; § Chuncheon Center, Korea Basic Science Institute, Chuncheon, Kangwon-do 200-701, Korea; ¶ Gwangju Center, Korea Basic Science Institute, Gwangju 500-757, Korea; and || Department of Chemistry and National Creative Research Initiative Center, Korea Advanced Institute of Science and Technology, Yusung-gu, Daejeon 305-701, Korea Submitted September 1, 2005, and accepted for publication November 16, 2005
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.R^sup 1^ and R^sup 2^ values were determined by fitting peak volumes to a two-parameter single exponential decay using MATLAB
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