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(1997) have shown that the rate of reactivation of GR that had been previously unfolded by GdnHCl is not altered by addition of FAD
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- 4 µM) were incubated for 90 min in the absence or in the presence of different GdnHCl concentrations and applied onto a Superdex 200 HR column (Pharmacia, Uppsala, Sweden), previously equilibrated with 20 mM Tris-HCl, at pH 7
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. Interestingly, no significant spectral changes were detected up to 1 M GdnHCl, whereas an increase in reactivity of one thiol group of GR to DTNB was observed at this concentration of GdnHCl (Fig
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. The I [arrow right] A transition, which was referred to as the dominant unfolding process above, began at around 70°C and the midpoint of the transition (T^sub 1/2^) occurred at ~84°C (see Table 1)
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